- Gru as Po
- Dr.Nefario as Master Shifu
- Margo as Master Tigress
- Tim the Minion as Master Monkey
- Bob the Minion as Crane
- Mark the Minion as Mantis
- Edith as Viper
- Mr. Perkins as Commander Vachir
- Eduardo as Tai Lung
- Vector as Lord Shen
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