- Paul Morgan as Mickey Mouse
- Meryl Morgan as Daisy Duck
- Clay Wheeler as Donald Duck
- Adam Feller as Goofy
- Jackie Drake as Minnie Mouse
- Vincent as Casey Junior
- Nurse Kelly as Clarabelle Cow
- Rodeo as Oswald the Lucky Rabbit
- Emma Wheeler as Clara Cluck
- Earl Granger as Scrooge McDuck
- Lucy Granger as Ortensia
- Doc D. Simmons as Professor Ludwig Von Drake
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