- Timber as Mr. Conductor
- Candy Kong as Stacy Jones
- Donkey Kong as Schemer
- Diddy Kong as Matt
- Dixie Kong as Tanya
- Tiptup as Dan
- Pipsy as Becky
- Tiny Kong as Kara
- Banjo and Krunch as Tex and Rex
- Bumper as Tito
- Wizpig as Mr. Conductor's Evil Twin
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