- James Bond as Peter Griffin
- Giacinta Johnson as Dr. Amanda Rebecca
- Gustav Graves as Stewie Griffin
- Miranda Frost as Connie D'Amico
- Zao as Chris Griffin
- M as Brian Griffin
- Colonel Moon as Dr. Hartman
- General Moon as Mayor Adam West
- Q as Cleveland Brown
- Charles Robinson as Joe Swanson
- Peaceful Fountains of Desire as Meg Griffin
- Raoul as Tom Tucker
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