- James Bond as Mario
- Giacinta Johnson as Princess Daisy
- Gustav Graves as Bowser
- Miranda Frost as Wendy O. Koopa
- Zao as Yoshi
- M as Luigi
- Colonel Moon as Wario
- General Moon as Toadsworth
- Q as Toad
- Charles Robinson as Donkey Kong
- Peaceful Fountains of Desire as Toadette
- Raoul as Mallow
- Miss Moneypenny as Princess Rosalina
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