- Donald Duck (from Mickey Mouse) as Buzz Lightyear
- Brainy (from The Smurfs) as XR
- Pumbaa (from The Lion King) as Booster
- Glimmer (from Tinkerbell) as Mira Nova
- Minister of Autumn (from Tinkerbell) as Commander Nebula
- Randall (from Monsters, Inc.) as Warp Darkmatter
- The Smurfs (from The Smurfs) as Little Green Men
- Agent Z as Himself
- Darth Vader (from Star Wars) as Evil Emperor Zurg
- Stone (from Tinkerbell and the Lost Treasure) as Space Rangers
- Shenzi, Banzai, and Ed (from The Lion King) as Grubs
- Other Hyenas (from The Lion King) as Brain Pods
- Pirates (from Peter Pan) as Hornets
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