- Big Bird as Phineas Flynn
- Baby Bear as Ferb Fletcher
- Betty Lou as Candace Flynn
- Alfred Duck as Perry the Platypus
- Iago the Donkey as Dr. Doofenshmirtz
- Zoe as Isabella Garcia-Shapiro
- Elmo as Baljeet
- Cookie Monster as Buford van Stomm
- Ernie as Jeremy Johnson
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