• Doc Holiday as Kermit the Frog
  • Kate Elder as Annie Sue Pig
  • Wyatt Earp as Rizzo the Rat
  • Ike Clanton as Gonzo the Great
  • The Kid as Dr. Teeth
  • Clum as Jim Henson
  • Bartlett as Dr. Bunsen Honeydew
  • Tombstone Sheriff John Behan as Sam the Eagle
  • Virgil Earp as Walter
  • James Earp as Rowlf the Dog
  • Mattie Earp as Miss Piggy
  • Alley Earp as Janice
  • Frank McLowery as Floyd Pepper
  • Concha as Wanda
  • Morgan Earp as Fozzie Bear

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