- Peter "Pete" LaFleur as Mario
- White Goodman as Bowser
- Katherine "Kate" Veatch as Princess Daisy
- Patches O'Houlihan as Luigi
- Young Patches as Toad
- Justin Redman as Yoshi
- Gordon Pibb as Toadsworth
- Steve "the Pirate" Cowan as Oogtar
- Owen Dittman as Wario
- Dwight Baumgarten as Waluigi
- Fran as Princess Rosalina
- Me'Shell Jones as Donkey Kong
- Cotton McKnight as Diddy Kong
- Pepper Brooks as Bowser Jr.
- The Dodgeball chancellor as Boo
- Amber as Princess Peach
- Derek as Detective Herlock Solmes
- Mr. Ralph as Larry Koopa
- Joyce as Toadette
- and more
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