• Ricardo Quintana as Mario
  • Glenda Crawford as Princess Daisy
  • Carmen Miranda as Princess Peach
  • Binnie Crawford as Princess Rosalina
  • Casiano as Luigi
  • Don Diego Quintana as Toad
  • Helen Carson as Birdo
  • Tito Acuna as Yoshi
  • Esteban as Wario
  • Jimmy Blake as Waluigi
  • Sebastian as Donkey Kong
  • Panchito as Diddy Kong
  • Dr. Arturo Padilla as Larry Koopa
  • The Nicholas Brothers (Specialty Dancers) as Oogtar and Detective Herlock Solmes

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