- Lt. Commander Thomas "Tom" Dodge as Kermit the Frog
- Lt. Emily Lake as Miss Piggy
- Lt. Martin T. "Marty" Pascal as Dr. Bunsen Honeydew
- Chief of the Boat as Beaker
- Rear Admiral Yancy Graham as Sweetums
- Commander Carl Knox as Dr. Teeth
- Culinary Specialist 2nd Class Buckman as Animal
- Fireman Niitro "Mike" as Rowlf the Dog
- Planesman 1st Class Jefferson "R.J." Jackson as Gonzo the Great
- Seaman Stanley "Spots" Sylvesterson as Rizzo the Rat
- Engineman 1st Class Brad Stepanak as Scooter
- Sonarman 2nd Class E.T. "Sonar" Lovacelli as Statler
- Vice Admiral Dean Winslow as Waldorf
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