- Lt. Commander Thomas "Tom" Dodge as Buzz Lightyear
- Lt. Emily Lake as Jessie the Cowgirl
- Lt. Martin T. "Marty" Pascal as Woody
- Chief of the Boat as Rex
- Rear Admiral Yancy Graham as Hamm
- Commander Carl Knox as Andy Davis
- Culinary Specialist 2nd Class Buckman as Stinky Pete
- Fireman Niitro "Mike" as Bullseye
- Planesman 1st Class Jefferson "R.J." Jackson as Wheezy
- Seaman Stanley "Spots" Sylvesterson as Lotso Huggin Bear
- Engineman 1st Class Brad Stepanak as Slinky Dog
- Sonarman 2nd Class E.T. "Sonar" Lovacelli as Sid Phillips
- Vice Admiral Dean Winslow as Mr. Potato Head
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