- Dr. John Dolittle as Kristoff
- Grandpa Archer Dolittle as Sven
- Dr. Mark Weller as Olaf
- Calloway as Pabbie
- Lucky the Dog as Young Sven
- Rodney the Guinea Pig as The King of Arendelle
- Dr. Gene "Geno" Reiss as Gothi
- Lisa Dolittle as Anna
- Baby Tiger as Young Kristoff
- Siberian Husky as Marshmallow
- Maya Dolittle as Elsa
- Skunk as Prince Hans
- and more
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