- Dr. John Dolittle as Wallace
- Grandpa Archer Dolittle as Hutch
- Dr. Mark Weller as Gromit
- Calloway as Mr. Growbag
- Lucky the Dog as Mr. Mulch
- Rodney the Guinea Pig as PC Macintosh
- Lisa Dolittle as Lady Tottington
- Dr. Fish as Shaun the Sheep
- Old Beagle as The Wrong Trousers
- Baby Tiger as The Moon Machine
- Maya Dolittle as Fluffles
- and more
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