• Dr. John Dolittle as Mario
  • Charisse Dolittle as Princess Daisy
  • Joe Potter as Toad
  • Riley as Luigi
  • Lisa Dolittle as Princess Rosalina
  • Maya Dolittle as Princess Peach
  • Eric as Yoshi
  • Logger as Oogtar
  • Archie as Wario
  • Ava as Toadette
  • Drunk Monkey as Donkey Kong
  • Lucky Dolittle as Toadsworth
  • Alligator as Diddy Kong
  • Giraffe as Detective Herlock Solmes
  • and more

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