- Michael Shayne as Mario
- Joanne La Marr as Princess Peach
- Connie Earle as Birdo
- Inspector Pierson as Yoshi
- Lynne Evans as Princess Daisy
- Max Allaron as Luigi
- Otto Kahn as Donkey Kong
- Julian Davis as Diddy Kong
- Hal Brennon as Mallow
- David Earle as Toad
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