- Rufus T. Firefly as Mario
- Pinky as Yoshi
- Chicolini as Luigi
- Lt. Bob Roland as Diddy Kong
- Mrs. Gloria Teasdale as Lady Lima
- Ambassador Trentino of Sylvania as Donkey Kong
- Vera Marcal as Princess Peach
- Lemonade Vendor as Toadsworth
- Former President Zander as Wario
- Former Secretary of War as Bowser
- First Minister of Finance as Oogtar
- Second Minister of Finance as Toad
- Sylvanian Agitator as Smithy
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