- Jerry Shaw as Mario
- Ethan Shaw as Detective Herlock Solmes
- Rachel Holloman as Princess Peach
- ARIIA as Wendy O. Koopa
- Zoe Perez as Toadette
- Teenage Page as Princess Daisy
- George Callister as Donkey Kong
- Major William Bowman as Toadsworth
- Toby Grant as Wario
- Tom Morgan as Waluigi
- Ranim Khalid as Oogtar
- Sam Holloman as Luigi
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