• Bill Chandler as Mario
  • Connie Allenbury as Princess Peach
  • Gladys Benton as Birdo
  • Warren Haggerty as Yoshi
  • J.B. Allenbury as Luigi
  • Spike Dolan as Diddy Kong
  • Curtis Farwood as Wario
  • Hector Boswell as Toad
  • Joe as Donkey Kong
  • Mary as Princess Daisy
  • Barbara Norvell as Toadette
  • Homer Henshaw as Toadsworth
  • Mrs. Burns Norvell as Princess Rosalina

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