• Ruth Wiley as Princess Peach
  • Dick Carver as Mario
  • John Wiley as Luigi
  • Appuhamy as Wario
  • Chief Planter Atkinson as Toadsworth
  • Doctor Pereira as Diddy Kong
  • Mrs. Lakin as Princess Daisy
  • Planter Gordon Gregory as Waluigi
  • Planter Chisholm as Nabbit
  • Rayna as Birdo

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