- Ruth Wiley as Princess Peach
- Dick Carver as Mario
- John Wiley as Luigi
- Appuhamy as Wario
- Chief Planter Atkinson as Toadsworth
- Doctor Pereira as Diddy Kong
- Mrs. Lakin as Princess Daisy
- Planter Gordon Gregory as Waluigi
- Planter Chisholm as Nabbit
- Rayna as Birdo
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