- "Slim" Hiller/Erin Shleeter as Pauline
- Mitch Hiller as Mario
- Gracie Hiller as Birdo
- Ginny as Princess Peach
- Joe as Luigi
- Robbie as Yoshi
- Jupiter as Toad
- Mr. Hiller as Donkey Kong
- Mrs. Hiller as Toadette
- Jim Toller as Wario
- Phil as Waluigi
- Instructor as Detective Herlock Solmes
- Homeowner as Toadsworth
- Teddy as Larry Koopa
- FBI Agent #1 as Bowser
- FBI Agent #2 as Bowser Jr.
- FBI Agent #3 as Morton Koopa, Jr.
- Desk Sergeant as Diddy Kong
- Mitch's Young Blonde as Princess Daisy
- Mitch's Assistant as Princess Rosalina
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