• Frank Morris as Mario
  • Clarence Anglin as Luigi
  • John Anglin as Detective Herlock Solmes
  • Charley Butts as Yoshi
  • Warden Paul J. Madigan as Toadsworth
  • English as Donkey Kong
  • Litmus as Toad
  • Chester "Doc" Balton as Mallow
  • Wolf Grace as Diddy Kong
  • Inmate as Wario
  • Beck as Oogtar

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