• Cornelius as Mario
  • Zira as Princess Peach
  • Dr. Lewis Dixon as Yoshi
  • Dr. Stephanie Branton as Princess Daisy
  • Dr. Otto Hasslein as Bowser
  • President as Toadsworth
  • Dr. Milo as Luigi
  • E-1 as Troff
  • E-2 as Scoff
  • Chairman as Oogtar
  • General Winthrop as Mallow
  • Aide as Donkey Kong
  • Lawyer as Smithy
  • Cardinal as Waluigi

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