- Cornelius as Kermit the Frog
- Zira as Miss Piggy
- Dr. Lewis Dixon as Rizzo the Rat
- Dr. Stephanie Branton as Camilla the Chicken
- Dr. Otto Hasslein as Uncle Deadly
- President as Jim Henson
- Dr. Milo as Gonzo the Great
- E-1 as Dr. Bunsen Honeydew
- E-2 as Beaker
- Chairman as Floyd Pepper
- General Winthrop as Fozzie Bear
- Aide as Sam the Eagle
- Lawyer as Lips
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