- Ben Lassiter as Mario
- Beth Drury as Princess Peach
- Martha Drury as Princess Daisy
- Abbey Lassiter as Toadette
- Lt. Jamison as Toadsworth
- Trooper Vogel as Donkey Kong
- Nelson Walker as Wario
- Lt. Weeks as Mallow
- Trooper Travis as Yoshi
- Corporal Wheeler as Bowser Jr.
- Sgt. Doyle as Waluigi
- Capt. Howard Poole as Harold
- Troober Burch as Baby Mario
- Mrs. Kate Fenniman as Princess Rosalina
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