- Chunk Squarey (from Rolie Polie Olie) as Jackman Wolf
- Homestar Runner (from Homestar Runner) as Big Bird
- Big Mouth (from Peg + Cat) as Cookie Monster
- The Cat in the Hat (from The Wubbulous World of Dr. Seuss) as Telly Monster
- Richard (from Peg + Cat) as Elmo
- Peg (from Peg + Cat) as Zoe
- The Ferocious Beast (from Maggie and the Ferocious Beast) as Snuffy
- Clover (from Sofia the First) as Hoots the Owl
- Phineas Flynn (from Phineas and Ferb) as Bert
- Ferb Fletcher (from Phineas and Ferb) as Ernie
Chunk Squarey: Everybody twist! Now twist again!
Everyone: Let's twist again,
Like we did last summer!
The Cat in the Hat: Yeah! Come on!
Everyone: Yeaaah, let's twist again,
Like we did last year!
Peg: Oh, boy! Hey, this is fun!
Everyone: Do you remember when,
Things were really hummin',
Yeaaaah, let's twist again,
(Peg: All right!)
Twistin' time is here!