- Dr. Teeth (from The Muppets) as Guy Smiley
- The Announcer (from Homestar Runner) as The Announcer
- Sofia (from Sofia the First) as Prairie Dawn
- Fozzie Bear (from The Muppets) as Bert
Fozzie Bear: That's a nice song, Kermit, but what letter comes next? Let's see, uh, A, B, C, D...
The Announcer: And now, it's time for that great new game show "What's My Letter?". And here's the host of the show, Dr. Teeth.
Dr. Teeth: Thank you! Thank you! And now, before we begin our game, let me tell you that today's letter is the letter "E". Remember, that's the letter "E". And now, let's meet our first contestant, a lovely young lady, Sofia.
Sofia: Thank you. It is sincerely a pleasure to be here Dr. Teeth.
Dr. Teeth: And now, Sofia, here is your question. In the alphabet, what letter comes after the letter "D"?
Sofia: Oh mercy, I am afraid I do not know.
Dr. Teeth: Think now Sofia it comes after D and just before F.
Sofia: Uh-huh. Oh, what a puzzler.
Dr. Teeth: Sometimes it sounds like Eh as in egg, elbow, and elephant.
Sofia: Egg, elbow, elephant. Oh, I have not the faintest.
Dr. Teeth: Other times it sounds like eee, as in eat, Easter, and evening.
Sofia: This is a very difficult question.
Dr. Teeth: Time is running out. Remember if you answer correctly, you will win our grand prize, this delightful pet mouse.
Sofia: A mouse??!! EEEEEEEEEEEEEEEEE!!!!!!!!
Dr. Teeth: That's absolutely right! Yes! E is the letter right after D, and here is your mouse!
Dr. Teeth: That's all the time we have for today, get off the table Sofia. Tune in next time for another exciting game of What's My Letter?
Sofia: EEEEEEEEE! EEEEEEEEEE!