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- Fanboy as Batman
- Chum Chum as Robin
- Yo as Batgirl
- Oz as Alfred
- Janitor Poopatine as Joker
- Professor Flan as Penguin
- Francine as Catwoman
- Kyle The Conjurer as James Gordon
- Global Warmer as Riddler
- Boog as Two-Face
- Sigmund the Sorcerer as Mad Hatter
- Marsha as Poison Ivy