- Yo as Mrs. Tiggy-Winkle
- Fanboy as Mr. Jeremy Fisher
- Nancy Pancy as Lucie
- Kyle Bloodworth-Thomason as Sir Isaac Newton
- Chum Chum as Alderman Ptolemy Tortoise
- Duke as Peter Rabbit
- Michael Johnson as Benjamin Bunny
- Janitor Russ Poopatine as Mr. McGregor
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