- Humphrey as Mario
- Agatha Floud as Princess Rosalina
- Cart Belknap as Luigi
- Mike Floud as Toad
- Effie Floud as Princess Daisy
- George Van Basingwell as Yoshi
- Sir Wembley as Donkey Kong
- Wampum as Diddy Kong
- Teddy Roosevelt as Bowser
- Lady Maude as Princess Peach
- Rosalind as Birdo
- Twombley as Bowser Jr.
- Wong as Wario
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