- Humphrey as Mickey Mouse
- Agatha Floud as Daisy Duck
- Cart Belknap as Professor Ludwig Von Drake
- Mike Floud as Donald Duck
- Effie Floud as Minnie Mouse
- George Van Basingwell as Pluto
- Sir Wembley as Mortimer Mouse
- Wampum as Oswald the Lucky Rabbit
- Teddy Roosevelt as Pete
- Lady Maude as Clarabelle Cow
- Rosalind as Clara Cluck
- Wong as Scrooge McDuck
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