- Twilight Sparkle (from Equestria Girls) as John Lennon
- Rarity (from Equestria Girls) as Paul McCartney
- Fluttershy and Pinkie Pie as an extra (from Equestria Girls) as Ringo Starr
- Applejack and Rainbow Dash as an extra (from Equestria Girls) as George Harrison
- Neptuna (The Hydronauts) as Captain Fred
- Patricia (from Spliced) as Jeremy Hillary Boob, Ph.D.
- Ice Queen (from Adventure Time) as Chief Blue Meanie
- Lapis Lazuli (from Steven Universe) as Max
- ??? as Storm Bloopers
- ??? as Apple Blonkers
- ??? as Clows
- ??? as Snapping Turtle Terps
- ??? as Butterfly Stompers
- ??? as Flying Glove
- ??? as Lord Mayor
- ??? as Pepperland Citizens
- ??? as Officer
- ???, Sirens (from Ice Age 4: Continental Drift), ???, ??? and ??? as Sea Monsters
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