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- Daisy as Basil
- Canky Kong as Dawson
- Mario as Olivia
- Rosalina as Hiram
- Wario as Ms. Judson
- Wendy O. Koopa as Fidget
- Rouge (from Sonic) as Ratigan
- Peach as Fidget are disguised with baby boy
- Birdo as Bartholomew
- Baragon (from Godzilla) as Felicia
- Splinter (from TMNT) as Queen Victoria