- Phineas as Buster Bunny
- Isabella as Babs Bunny
- Jeremy as Plucky Duck
- Ferb as Hamton J. Pig
- Candace as Shirley the Loon
- Gretchen as Fifi la Fume
- Suzy as Montana Max
- Professor Poofenplotz as Elmyra Duff
- Dr. Doofenshmirtz as Dr. Gene Splicer
- and more
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