- Yakko as Thomas
- Ralph as Henry
- Runt as Duck
- King Acorn (from Sonic The Hedgehog) as Sir Topham Hatt
- Dr. Otto as Edward (does not speak)
- Pesto as James (cameo)
- Wakko as Percy (cameo)
- Buster Bunny (from Tiny Toon Adventures) as Skarloey (stock footage cameo)
- Hamton J. Pig (from Tiny Toon Adventures) as Rheneas (stock footage cameo)
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