- Lane Bellamy as Princess Peach
- Fielding Carlisle as Mario
- Sheriff Titus Semple as Donkey Kong
- Lute Mae Sanders as Birdo
- Dan Reynolds as Yoshi
- Annabelle Weldon as Princess Daisy
- Dr. Waterson as Toadsworth
- Millie as Toadette
- Pete Ladas as Wario
- Gracie as Princess Rosalina
- Boatright as Oogtar
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