- Irwin "Fletch" Fletcher as Mario
- Hamilton "Ham" Johnson as Luigi
- Becky Culpepper as Princess Daisy
- Jimmy Lee Farnsworth as Toad
- Frank Walker as Yoshi
- Ben Dover as Diddy Kong
- Sheriff as Bowser
- Calculus Entropy as Bowser Jr.
- Marvil Gillet as Donkey Kong
- Amanda Ray Ross as Pauline
- Ku Klux Klan Leader as Larry Koopa
- Phil as Wario
- Bly Bio Manager as Waluigi
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