- Mario as Thomas
- Donkey Kong as Gordon
- Rabbit as James
- Diddy Kong as Henry (non-speaking role)
- The Mayor of Townsville as Sir Topham Hatt (non-speaking role)
- Blossom and Bubbles as Annie and Clarabel (non-speaking role)
- Sonic as Bertie (cameo)
- George Carlin as Narrator
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