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- Mario as Thomas
- Mr. Krabs as Edward
- Donkey Kong as Gordon
- Yoshi as Percy
- The Mayor of Townsville as Sir Topham Hatt
- Diddy Kong as Henry (non-speaking role)
- Rabbit as James (non-speaking role)
- Blossom and Bubbles as Annie and Clarabel (non-speaking role)
- George Carlin as Narrator