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- Rabbit as James
- Yoshi as Percy
- Cheatsy Koopa and Bully Koopa as 'Arry and Bert
- The Mayor of Townsville as Sir Topham Hatt
- Mario as Thomas
- Diddy Kong as Henry
- Donkey Kong as Gordon
- Luigi as Duck
- Blossom and Bubbles as Annie and Clarabel
- Alec Baldwin as Narrator