- Hip and Hop as Bill and Ben
- Numbuh 5 as Mavis
- Funky Kong as Salty
- The Mayor of Townsville as Sir Topham Hatt
- Mario as Thomas (cameo)
- Mr. Krabs as Edward (cameo)
- Diddy Kong as Henry (cameo)
- Donkey Kong as Gordon (cameo)
- Rabbit as James (cameo)
- Yoshi as Percy (cameo)
- Alec Baldwin as Narrator
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