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- Mario as Thomas
- Donkey Kong as Gordon
- Rabbit as James
- Boog as Spencer
- The Mayor of Townsville as Sir Topham Hatt
- T.K. Takashi and Kari Kamiya as The Duke and Duchess of Boxford
- Mr. Krabs as Edward (cameo)
- Diddy Kong as Henry (cameo)
- Yoshi as Percy (cameo)
- Princess Toadstool as Emily (cameo)
- Blossom and Bubbles as Annie and Clarabel (cameo)
- Michael Brandon as Narrator