- Mario as Thomas
- Mr. Krabs as Edward
- Diddy Kong as Henry
- Rabbit as James
- Toad as Toby
- The Mayor of Townsville as Sir Topham Hatt
- Donkey Kong as Gordon (cameo)
- Yoshi as Percy (cameo)
- Luigi as Duck (cameo)
- Blossom and Bubbles as Annie and Clarabel (cameo)
- Buttercup as Henrietta
- Alec Baldwin as Narrator
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