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- Mario as Thomas
- Yoshi as Percy
- Tigger as Arthur
- The Mayor of Townsville as Sir Topham Hatt
- Luigi as Duck (does not speak)
- Kirby as Harvey (does not speak)
- Diddy Kong as Henry (cameo)
- Donkey Kong as Gordon (cameo)
- Rabbit as James (cameo)
- Funky Kong as Salty (cameo)
- Pelipper as Butch (cameo)