- Mario as Thomas
- Tigger as Arthur
- The Mayor of Townsville as Sir Topham Hatt
- Yoshi as Percy (does not speak)
- Diddy Kong as Henry (cameo)
- Donkey Kong as Gordon (cameo)
- Luigi as Duck (cameo)
- Princess Toadstool as Emily (cameo)
- Larry The Lobster as Murdoch (cameo)
- Boog as Spencer (cameo)
- Blossom and Bubbles as Annie and Clarabel (cameo)
- Squidward as Cranky (cameo)
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