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- Mario as Thomas
- Diddy Kong as Henry
- Donkey Kong as Gordon
- Rabbit as James
- Yoshi as Percy
- Sonic as Bertie
- Squidward as Cranky
- The Mayor of Townsville as Sir Topham Hatt
- Pooh as Donald (cameo)
- Taillow as Tiger Moth (cameo)
- Mayor's Wife as Dowager Hatt (cameo)
- Alec Baldwin as Narrator