- Kristoff as Dr. John Dolittke
- Sven as Grandpa Arch Dolittle
- Olaf as Dr. Mark Weller
- Pabbie as Calloway
- Young Sven as Lucky the Dog
- The King of Arendelle as Rodney the Guinea Pig
- Gothi as Dr. Gene "Geno" Reiss
- Anna as Lisa Dolittle
- Young Kristoff as Baby Tiger
- Marshmallow as Siberian Husky
- Elsa as Maya Dolittle
- Prince Hans as Skunk
- and more
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