- Jason Nesmith as Mario
- Gwen DeMarco as Princess Daisy
- Alexander Dane as Toad
- Fred Kwan as Wario
- Guy Fleegman as Toadsworth
- Tommy Webber/Lt. Laredo as Luigi
- Mathesar as Oogtar
- Gen. Roth'h'ar Sarris as Bowser
- Brandon as Waluigi
- Laliari as Princess Peach
- Quellek as Donkey Kong
- Kyle as Diddy Kong
- Neru as Nabbit
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