- Geoffrey Giraffe as Phineas Flynn
- M Monkey as Ferb Fletcher
- K Kangaroo as Isabella Garcia-Shapiro
- N Newt as Baljieet
- Junior the Buffalo (From Home on the Range) as Buford Van Stomm
- D Deer as Candace Flynn
- B Bear as Major Monogram
- G Giraffe as Perry the Platypus
- F Fish as Stacy Hirano
- A Alligator as Jeremy Johnson
- O Ostrich as Vanessa Doofenshmirtz
- Lucy Van Pelt (From Peanuts) as Suzy Johnson
- Tai Lung (From Kung Fu Panda) as Dr. Heinz Doofenshmirtz
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