- Johnny Blaze / Ghost Rider as Mario
- Roxanne Simpson as Princess Rosalina
- Mephistopheles as Luigi
- Blackheart / Legion as Toad
- Carter Slade / Caretaker as Yoshi
- Young Johnny Blaze as Baby Mario
- Young Roxanne Simpson as Princess Peach
- Barton Blaze as Waluigi
- Mack as Oogtar
- Team Blaze as Detective Herlock Solmes
- Captain Dolan as Donkey Kong
- Girl in Alley as Princess Daisy
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